mutagenesis of a huge gene

benan dincturk knben at
Mon Jun 24 13:55:26 EST 1996

	I am using Clontech's site-directed mutagenesis kit to mutate a 4 
kb gene. With the vector it is more than 8 kb. Has anybody tried it? They 
say people did it with a 14 kb construct. Does anybody know any tricks or 
can anybody suggest any other method?

Benan Dincturk
knben at

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