x-gal plates for yeast

jstrassw at opal.tufts.edu jstrassw at opal.tufts.edu
Tue Jun 25 10:19:05 EST 1996

In article <31CBF195.1BF at lovelace.infobiogen.fr>, Namy Olivier <namy at lovelace.infobiogen.fr> writes:
> hello,
> To screen blue colonies with x-gal plates It's better to use synthetic 
> medium (see maniatis) than minimal medium. Don't forget to use 
> phosphate buffer because x-gal doesn't work for yeast at the normal acidic 
> pH of minimal medium; therefore a neutral pH medium is used (but many 
> yeast strains will not not grow very well at this pH...)
> Olivier Namy (France).

We successfully select first on glucose (and nutritional marker plates) and
replica-plate to Xgal/Galactose plates which induce our proteins of interest
(they are under control og GAL UAS, instead of the stasndard CYC1 or ADH


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