x-gal plates for yeast

jstrassw at opal.tufts.edu jstrassw at opal.tufts.edu
Tue Jun 25 10:19:05 EST 1996




In article <31CBF195.1BF at lovelace.infobiogen.fr>, Namy Olivier <namy at lovelace.infobiogen.fr> writes:
> hello,
> To screen blue colonies with x-gal plates It's better to use synthetic 
> medium (see maniatis) than minimal medium. Don't forget to use 
> phosphate buffer because x-gal doesn't work for yeast at the normal acidic 
> pH of minimal medium; therefore a neutral pH medium is used (but many 
> yeast strains will not not grow very well at this pH...)
> Olivier Namy (France).

We successfully select first on glucose (and nutritional marker plates) and
replica-plate to Xgal/Galactose plates which induce our proteins of interest
(they are under control og GAL UAS, instead of the stasndard CYC1 or ADH
promoters.

John





More information about the Methods mailing list