More question about gel-shift assay
cve at ifm.mh-hannover.de
Mon Jun 24 05:33:10 EST 1996
In article <3DIJ99$xL1 at bbs.life.nthu.edu.tw>,
klebs.bbs at bbs.life.nthu.edu.tw (Leo) wrote:
> It always yielded
> a smear pattern. When you increase the protein concentration,
> the DNA/protein complex just stuck in the wells.
500 bp is a pretty large probe. Try decreasing the acrylamide percentage
of your gel. This solved my "smear" problem. You could also try a 0.5x TBE
-- "Two bands or not two bands? - THAT is the question!"
Institute for Molecular Biology cve at ifm.mh-hannover.de
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