More question about gel-shift assay

[Christian Velten]

In article <3DIJ99$xL1 at bbs.life.nthu.edu.tw>,
klebs.bbs at bbs.life.nthu.edu.tw (Leo) wrote:

> It always yielded
>  a smear pattern. When you increase the  protein concentration,
>  the DNA/protein complex just stuck in the wells.

500 bp is a pretty large probe. Try decreasing the acrylamide percentage
of your gel. This solved my "smear" problem. You could also try a 0.5x TBE
running buffer.


Christian

--      "Two bands or not two bands? - THAT is the question!"

Christian Velten
Institute for Molecular Biology              cve at ifm.mh-hannover.de
Medical School Hannover                      Compuserve:100736,2071
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