3' RACE Difficulties

Nanto Cielens nanto.cielens at flinders.edu.au
Tue Jun 25 11:22:05 EST 1996


I am having difficulties obtaining a 3'RACE product!
I know about 120 bases of sequence and have designed a primer to this and a 3' adapter. 
I am also using Advantage Taq Polymerase from CLONTECH since I expect the product to be 
approximately 1-2kb in size. 
With this, I obtained junk and single primer annealing products only. 
I then designed nested primers and tried second round amplification and different 
annealing temperatures..no luck. Any trouble shooting suggestions?? 
Much appreciated.

lyn.kilpatrick at flinders.edu.au



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