Cleanup of short PCR Product
guymer at innogenetics.be
Tue Jun 25 03:37:07 EST 1996
michaelm at vcp1.vcp.monash.edu.au (Michael J. McLeish) wrote:
>I have a 90 bp PCR fragment that I would like to clean up, sufficiently for
>direct sequencing. Most resins seem to have extremely low efficiency for
>fragments of this size. Any ideas on how to go about this?
>Michael J. McLeish Ph.D. : Michael_m at vcp.monash.edu.au
>Department of Medicinal Chemistry
>Victorian College of Pharmacy
>Parkville VIC 3052
Amersham LIFE SCIENCE has a new kit: Reagent Pack for use with Sequenase
PCR Product Sequencing Kit. Product number: US70995
This kit is a new method for preparing the products of PCR for
sequencing. This method requires no purification or separation steps.
This kit uses two enzymes which effectively remove the excess of dNTP's
and primers from the PCR mix.
I haven't tried it, yet.
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