Cleanup of short PCR Product
skoufs at minerva.cis.yale.edu
Tue Jun 25 11:20:18 EST 1996
On Tue, 25 Jun 1996, Michael J. McLeish wrote:
> I have a 90 bp PCR fragment that I would like to clean up, sufficiently for
> direct sequencing. Most resins seem to have extremely low efficiency for
> fragments of this size. Any ideas on how to go about this?
I have been succesfully cleaning small PCR fragments (70-100 nt) in the
-Run on a 4% (3:1 Nusieve:SeaKemp ,tms FMC) gel in 1x TBE
-Use the Qiaquick (tm, Qiagen) gel elution columns
I never sequenced *PCR fragments* purified this way (I prefer to clone
them in TA-type vectors and then sequence); however, in the Qiagen protocol
it is mentioned that DNA cleaned this way is a good sequencing substrate.
(I have no affiliation with the above mentioned companies, just use their
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