nuclear extracts/footprinting

Bruce C. Kone, M.D. bkone at
Tue Jun 25 10:00:45 EST 1996

I'm having problems with in vitro DNAseI footprinting -  it may be my 
nuclear extracts (prepared without dialysis at end of the protocol).  
Does anyone have a sure-fire way to prepare nuclear extracts for 
footprinting?  Any new improvements of the in vitro footprinting method 
in Current Protocols?

Bruce C. Kone, M.D., FACP
Associate Professor of Internal Medicine
  and Integrative Biology
University of Texas Medical School at Houston

6431 Fannin, MSB 4.148
Houston, TX 77030
Telephone:  713-745-2505
FAX:  713-745-2505 or 713-794-1197

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