nuclear extracts/footprinting

Bruce C. Kone, M.D. bkone at heart.med.uth.tmc.edu
Tue Jun 25 10:00:45 EST 1996


I'm having problems with in vitro DNAseI footprinting -  it may be my 
nuclear extracts (prepared without dialysis at end of the protocol).  
Does anyone have a sure-fire way to prepare nuclear extracts for 
footprinting?  Any new improvements of the in vitro footprinting method 
in Current Protocols?

Thanks.
-- 
=============================================================
Bruce C. Kone, M.D., FACP
Associate Professor of Internal Medicine
  and Integrative Biology
University of Texas Medical School at Houston

6431 Fannin, MSB 4.148
Houston, TX 77030
Telephone:  713-745-2505
FAX:  713-745-2505 or 713-794-1197
Internet:  http://www.uth.tmc.edu/~bkone



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