[Q] ThermoSequenase aging
pmiguel at bilbo.bio.purdue.edu
Tue Jun 25 12:40:57 EST 1996
In article <email@example.com>,
kofoid at BIOLOGY.UTAH.EDU (Eric Kofoid) wrote:
> I began using Amersham's ThermoSequenase kit in February, and got
> excellent results with double stranded PCR templates. After a couple of
> Before I start the lengthy task of checking each reagent, can anybody
> offer any suggestions? Is this a particularly fragile enzyme? Do the
> termination mixes age unusually fast? Has anybody else noticed a short
> shelf life for this kit?
We keep our thermosequenase kit at 4 oC. Seems fine. The kit suggests
it may be kept at 4 oC for 3 months. Perhaps repeated freeze-thawing is
is the culprit.
thermosequenase is a thermostable polymerase engineered to be more
t7-like -- not the other way around. So it is quite thermostable.
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