in vitro transcription

Yiming Bao ybao at noble.org
Wed Jun 26 16:01:19 EST 1996


I had been doing in vitro transcription (T7) using a kit from Company A for some time without
any problems.  Recently, I used a fresh kit from this company and could not get any transcripts
from the same DNA template that worked before?  This same DNA template produces transcripts
when another kit from Company E is used?  So what could be wrong here?

Yiming Bao
Noble Foundation
YBAO at NOBLE.ORG




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