elauzier at fse.ulaval.ca
Wed Jun 26 14:48:12 EST 1996
When I use The Chomczynski and Sacchi method for RNA extraction...
1. The homogenizer will shear DNA, but why the latter goes to the
interface and/or the organic phase ? Since it does not shear the RNA, the
"sheared" DNA must be approximatly the same size. What affects the DNA
that doesn't affect the RNA ( Na acetate ?, phenol pH ? ... ) ?
2. When they say resuspend the pellet with 75% ethanol do they mean
vortex, just a little shake, good wash etc ? Is it a critical step and
Sorry again for all those basic questions but help is badly needed...
Edouard Lauzier, B.Sc. elauzier at fse.ulaval.ca
Physical Activity Science Laboratory (418) 656-2131 #2929
(Laval University) G1K 7P4
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