RNA Extraction... ( Still )
Edouard Lauzier
elauzier at fse.ulaval.ca
Wed Jun 26 11:21:16 EST 1996
I was wondering if the pH of the phenol can affect the efficienty of the
phenol/chloroform extraction...
I'm still stuck with low 260/280 ratio and was wondering if a lower pH
value for the phenol could interfere with the RNA and/or the quality of
the extraction, leading to impurities...
I measured betwwen 6.0 and 6.5 for the phenol when it should have been
7.6 I think...
Then again, can I trust a pH measure where pH paper is wet with a drop of
phenol who's so strong...
Thanks for anything that could be related near or far ...
--
Edouard Lauzier, B.Sc. elauzier at fse.ulaval.ca
Physical Activity Science Laboratory (418) 656-2131 #2929
(Laval University) G1K 7P4
CANADA
More information about the Methods
mailing list