inosine in PCR primers

Martin Goodson bapn4 at
Wed Jun 26 10:38:35 EST 1996

Dear all,
In order to design a set of PCR primers I need to know how inosine affects
the binding and Tm of oligos. For instance, is it OK to have an
inosine 4 bases away from the 3' end of the primer or should this
position be kept four-way degenerate? Has there been anything published
on this?
thanks in advance

martin g

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