HELP! VENT / Pfu amplification

DavidM7643 davidm7643 at aol.com
Thu Jun 27 00:06:43 EST 1996


A Nucleic Acids Research paper (Vol. 20, No. 14 pg 3551-3554, 1992)
documents that the 3' to 5' exo activity of these proof reading enzymes
will degrade the amplification oligos. Their solution is to use
phosphorothioate primers to protect the primer from exo degradation. If
you don't really need the fidelity (a marketing ploy?) stick with what
works. 



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