ThermoSequenase
Alexander Kraev
kraev at bc.biol.ethz.ch
Thu Jun 27 12:11:31 EST 1996
To the best of my knowledge it is the mutated Taq published in PNAS 92:
6339-6343 by Tabor and Richardson, with the addition of pyrophosphatase.
It can not be substituted for by the regular Taq, because it works with
ddNTP/dnNTP ratio of 1:100, while Taq requires ca. 100:1.
I hope this helps,
Alexander Kraev, PhD Internet:
kraev at bc.biol.ethz.ch
> Lab.of Biochemistry III Phone: 0041-1-632-31-47
> Swiss Federal Inst. Of Technology FAX: 0041-1-632-12-13
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> CH-8092 Zurich /Sasha/kraev.html
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