intensifying screens

H. ROYCHOWDHURY hroychow at NMSU.EDU
Thu Jun 27 10:22:19 EST 1996


On Wed, 26 Jun 1996, Stephanie Tucker wrote:

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> My best answer - 
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> The intensifying screens are activated when exposed to -70C 
> temperatures.  

Oh!???

You DO NOT need two of them, just put one on one side of 
> the gel, and a piece of film on the other side.  This procedure can be 
> substituted for merely developing 32P gels at room temp., with no 
> intensifying screen.  This may suffice if you have a screaming hot gel.  
> INtensifying screens are good if you are looking for something barely 
> there, or if you are using 35S.  Just remember, take the saran wrap OFF 
> when exposing 35S gels!
> 
Interesting! You suggest that the Saran wrap be removed before exposing 
35S gels, yet advise using an intensifier screen for it? 


> ST
> 
> > 
> > ============================================================================
> > 
> > Robyn Wallace
> > Department of Cytogenetics and Molecular Genetics         /\_/\
> > Womens and Childrens Hospital                            ( o o )
> > North Adelaide, South Australia  5006                     > - <
> > Ph  61 08 204 6442
> > Fax 61 08 204 7342
> > Email  rwallace at mad.adelaide.edu.au
> > 
> > ============================================================================
> > 
> > 
> > 
> > 
> > 
> > 
> 
> 


			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
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