inosine in PCR primers
Dr Richard Sallie
sallier at cygnus.uwa.edu.au
Thu Jun 27 08:24:14 EST 1996
There has been quite a bit published on this, mostly in Nucleic Acids
Research. One of the important issues is what the 4th position will be
used for: If it contains a RE site that will be important to yo later,
then use of inosine may be a problem, depending on the enzyme to be used.
Otherwise, it is generally OK to use inosine in this position.
In article <4qrlhr$3ok at infa.central.susx.ac.uk>, bapn4 at central.susx.ac.uk
(Martin Goodson) wrote:
> Dear all,
> In order to design a set of PCR primers I need to know how inosine affects
> the binding and Tm of oligos. For instance, is it OK to have an
> inosine 4 bases away from the 3' end of the primer or should this
> position be kept four-way degenerate? Has there been anything published
> on this?
> thanks in advance
> martin g
Richard Sallie MD FRACP
Consultant Physician and Hepatologist,
Perth, Western Australia
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