Lipidex-1000; cholesterol binding assay
sbe at biochem.usyd.edu.au
Fri Jun 28 05:20:55 EST 1996
We are trying to use Lipidex-1000 (obtained as a dry powder from
Sigma) to bind 3H-labelled cholesterol as part of a lipid-binding
We swell the powder in 0.01 M phosphate, wash 5x with MeOH, and then
wash back into phosphate. We find that it does not bind 3H-labelled
cholesterol after this treatment (despite a number of literature
reports claiming that this is possible).
Does anyone have any suggestions?
Dr Simon B Easterbrook-Smith Phone: (+) 612 351 3905
Department of Biochemistry FAX: (+) 612 351 4726
University of Sydney Email: sbe at biochem.usyd.edu.au
Sydney NSW 2006
Every complex question has a simple, straight-forward,
easy to understand, wrong, answer.
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