DNA/RNA dipsticks

Anne Gillen ez049617 at peseta.ucdavis.edu
Thu Jun 27 18:58:38 EST 1996


On 19 Jun 1996, Robert Chandler wrote:

> In article <9605188351.AA835113251 at uu1500.epitope.com>,
> jkellogg at epitope.com ("Jill Kellogg") wrote:
> 
> > >From Cisca:
> > >I am trying to find out what company makes DNA/RNA Dipsticks. I remember
> > >seeing an advert. on these strips of `paper' which you can load DNA/RNA onto
> > >the paper then (I think) has a colour change or something that indicates the 
> > >amount of DNA/RNA present in ug. Unfortunately I can't remember who makes
> > >them. I'd love to hear from anyone that can help. Thanks Cisca.
> > 
> >     Invitrogen (1-800-955-6288) makes DNA Dipstick.
> > 
> 
> They are indeed made by Invitrogen. I tried them when they came out
> (circa. 1989, I think) and they were essentially worthless. They may be
> better now, but I wouldn't rely too heavily on their accuracy if I were
> you.BTW, I actually did a whole series of dilutions against known RNA and
> DNA standards to reach this conclusion. Expensive, too.
> 
> George
> 
> 

We use them sucessfully to quantify DNA for PCR.  They only work for 
samples under 10ng/ul.  For higher concentrations we use a spec or 
fluorometer.

Anne



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