blue colonies-no Xgal
Michael H. Bracey
mbracey at unix1.sncc.lsu.edu
Fri Jun 28 00:20:16 EST 1996
I recently cloned some PCR products into InVitrogen's tailed vector and
transformed the ligation products into their SureShot Top10 cells which
are supposed to have a lacIq repressor. In the *absence* of both IPTG
and X-gal, I got back about 50% blue colonies. Is there a trivial
explanation for this that I'm overlooking? Where is the blue coming
from?
Emailed replies are especially welcome.
Thanks,
Michael
mbracey at unix1.sncc.lsu.edu
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