Enterokinase

WUSC wusc at gt350h.dev.uga.edu
Fri Jun 28 12:03:24 EST 1996


Hi, someone in the cyberspace experienced with ENTEROKINASE?
We are trying to cleave the fusion tag of a protein expressed
in E.coli with enterokinase (Invitrogen).  What we got was partial
(very diluted enzymes used) or complete (0.5 unit) degradation of
the expressed protein, even at low Temp.(15C).  There is no 
specificity with this enzyme in our experiments.  What could go
WRONG?
CAN SOMEONE HELP?   Thanks.
sc



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