nuclear run-on for low abundant RNA
cchughes at uci.edu
Fri Jun 28 15:20:50 EST 1996
In article <1996Jun28.180353.17694 at alw.nih.gov>, bernard at elsie.nci.nih.gov
(Bernard Murray) wrote:
> An alternative is to look at the level of hnRNA for your transcript.
(...as apposed to doing a run-off.)
Whoa...hang-on there. I haven't read the article yet but looking at the
level of hnRNA measures the *combined* rates of transcription *and*
processing. It is not necessarily a good substitute for a run-off as
regulation of processing seems to be an important rate limiting step for
some genes. See for example some of the recent literature on regulation
of processing and transcription of the IL-2 gene. We developed a PCR
method for comparing processed and non-processed RNA levels but could
never convince ourselves that we were measuring transcription rate per se.
Just something to bear in mind.
Dr. Chris C.W. Hughes
Dept. Mol. Biol. & Biochem.
Biol. Sci. II
Univ. California, Irvine cchughes at uci.edu
Irvine CA 92717 ph (714) 824 8771
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