poly A+ purification

Szabadkai Gyorgy SZABADKAI at PUSKIN.SOTE.HU
Fri Jun 28 09:47:38 EST 1996


Hi,

I hae a problem with poly A preparation from long mRNAs. I have an 
about 20 times enrichment for GAPDH, but the longer about 6.5 kb mRNA 
is almost completely lost after purification. I use NEB oligodT-
cellulose column for purification.
Any tips?

thanks,


szagyo



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