in situs with oligos?

Majumder lab jhmorris at utmdacc.mda.uth.tmc.edu
Fri Jun 28 13:31:24 EST 1996


Hi, I'm new.

We are doing in situ hybridization on sections (paraffin, 35S, emulsion,
etc.).  No problem, I've done them before.  Due to some difficulties
obtaining a positive control probe from another lab, we were thinking of
synthesizing oligos and labelling them for in situs.  Is this possible? 
Has anyone tried it and gotten it to work?

Thanks,
--Julia
-- 
jhmorris at utmdacc.mda.uth.tmc.edu or zzhao at utmdacc.mda.uth.tmc.edu
Dept. of Neuro-Oncology
M.D. Anderson Cancer Center
Houston, TX



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