Lactose instead of IPTG

Fiona Thornton fthornton at macollamh.ucd.ie
Fri Jun 28 04:52:35 EST 1996


I have noticed from the newsgroups recently that some people use lactose
instead of IPTG as an inducer of expression. I would like to try this out
with my pRSET vector. Can anyone out here tell me if this is advisable and
if so what kind of concentration of lactose to use for induction.

Thanks 
Cliff



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