Beginner with RNA extraction

HaroldD885 haroldd885 at aol.com
Sun Jun 30 00:34:53 EST 1996


In addition to protein contamination lowering the "ideal" 260/280, another
source that lowers this ration is phenol contamination.  Phenol has a
maxima around 270.  If 270 is higher than either your 260 or 280, then
that's what you have.  That's a worse case; lesser amounts will lead to a
flattening of the spectrum just after 260.  An additional precipitation
with ethanol usually gets rid of this.  This is why I always take a full
spectrum (220 to 350) of all nucleic acid samples.  The shape of the curve
tells a lot.



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