Begining with Baculovirus

Tracy Aquilla aquilla at salus.med.uvm.edu
Fri Jun 28 13:59:42 EST 1996


In Article <josh-2706961149550001 at 155.37.2.111>, josh at panda.uchc.edu (Josh
Goldstein) wrote:
>In article <4qbh2q$aie at mercury.hgmp.mrc.ac.uk>, mlush at hgmp.mrc.ac.uk (Mr.
>M.J. Lush) wrote:
>>         The girl on the next bench is working with Baculovirus...
>> the main problem turned out to be that you need to use a cell line
>> with a low passarge (Sp?) number.   This fact was not mentioned
>> in any of the protocols and only transpired from conversation 
>> with the tech support (eventually!).
>> -- 
>> Michael
>
>
>   Actually, we've been using baculovirus expression for a while, and tech
>support told us that for Sf9 cells, they have been through at least 100
>passages before they even send them out, and that passage number is not
>really such a big deal. 


I've had Sf9 and Sf21 cells that went through over 1000 passages with no
problems. I've cultured some of these insect cell lines for up to seven
years. IF they're properly maintained, passage number isn't really a problem.
Tracy



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