Methods June 1996 Archives by thread
Starting: Sat Jun 1 00:23:30 EST 1996
Ending: Sun Jun 30 21:27:33 EST 1996
Messages: 1191
- Help : detection of mononucleotide on TLC
Dima Klenchin
- condensation when pouring agarose plates
Trevor Bezdek
- Inducible Retroviral Expression Vector
Fergus R. McKenzie
- Help with Diefenbakia and Yucca Plant, Pls
Kerstin Hoef-Emden
- NT'L SEARCH, BUSINESS UNIT MANAGER, LIFE SCIENCES
mercedesco at sprynet.com
- phenol stories
PROUET
- TKO miniflourometer (long)
Alexander Kraev
- DNA ligation help
chunlin xin
- pCDNA3 Cloning Difficulties
Alexander Kraev
- anti-TGFbeta antibodies, etc.
Kimberly Martin
- Pouring sequencing gels the old fashion way
Kimberly Martin
- HELP: NORTHERN NOVICE
Kimberly Martin
- mouse derived cells equivalent to COS cells
Bernard Murray
- Bibliography software recommendations?
M. Zafri Humayun
- HELP: NORTHERN NOVICE
David C. Logan
- DNA ligation help
chunlin xin
- Native Protein Gel
michael reddy
- Native Protein Gel
michael reddy
- DNA ligation help
Sailesh Surapureddi
- Acrylamide Gel setting too quickly -- IDEAS??
Brian MacNevin
- Job Opening
LEE
- PLEASE VISIT: http://www.muetec.com/sgk/index.htm
David C. Logan
- H E L P ! !
ppadula at IMPSAT1.COM.AR
- mRNA extraction from Gram positive bacteria
James Graham
- DEAE-dextran transfection/divalent cations
N.P.Dantuma at biol.ruu.nl
- SEAP assay
Protein Chemistry Facility
- PCR from lambda - primer help
John Dixon
- pcr problem with gc rich sequences
gwen jenkins
- Cloning Vectors
Jason Curole
- Anyone using "the hook"?
Bernard Murray
- (none)
Dianne Emslie
- Autoradiography of PAGE gels - please help
Megan
- Long Pcr...Ecperience and Suggestions (2.5 KB from Plasmid + linker)
Pam Snyder
- DNA Primers
Pam Snyder
- MJ thermal cycler problems
Tay Hou Ngee Agnes
- ProK Digestion
JohnnyHugh
- WE NEED YOUR INPUT
Survey Administration
- How do you determine % homology vs. stringency in a Southern Blot?
Charles A Miller
- DNA/RNA Stain
Clay Comstock
- Help : Detection of nucleotide on TLC
Dae-Young Kim
- [Q] mechanism of formation of pancreatic tail cancer?
Han Bok Kim
- Murine T-cell line responsive to IL-12?
Dirk Seegert
- Clontech epidermal cDNA libraries
Nick Jacobsen
- Anyone using p123T-vector?
Akira Nabetani
- mechanism of formation of pancreatic tail cancer?
Hanbok Kim
- Subcloing PCR Products
jms93
- repetitive dna
joerg hauf
- NaBr gradients versus KBr
Inmaculada Garcia Robles
- how many bases does an RE cover?
John Brennand
- supplier for human serum
Sabine Hanelt
- supplier for human serum
John Brennand
- Vancomycin resistance
Richard Moldwin
- RNAse protection assays
Richard Rohan
- (no subject)
Tim Lansing
- PEG in ligations, why?
Troels Wind
- inverse PCR on cDNA: any protocol available ?
Eric Campeau
- ABI 377 sequencer
Greg Roland
- Thermal cyclers for less than $3,000?
Mary P. Remington
- Uni-directional deletions
Mary P. Remington
- expressing truncated proteins
Richard Waring
- immunoscope software
Heidi Moss
- HELP/glucose assay kit of Sigma #635
Toshio
- PCR getting smear
Paul Brian Scruggs
- Pharmacology Data Base.
Paul Brian Scruggs
- Mouse epithelial cell library in pJG4-5 Info Request.
Sourav Ghosh
- Precipitating bactriophage
DANIEL Y KIM
- subcloning
Michael Witcher
- Nuclear translocation. How fast does it happen?
mmgosink at facstaff.wisc.edu
- StratagenQuikChange
Michael Forte
- Vancomycin resistance
Johan Burger
- Persistant phenotypic differences (serial subculture)?
James Graham
- culture society disc group
BADIDION at aol.com
- Quantify DNA
I-ching Yu
- beta-galactosidase assays using microtitre plates
Tsichlis Lab
- RDA (?)
Tsichlis Lab
- Swiss 3T3 transfection
Didier Thomas
- DEAE-dextran transfection/divalent cations
Graham Dellaire
- Microinjection of Murine ES cells... any experiences? What is involved?
Graham Dellaire
- How many ug of RNA for differential display
masato Kinoshita
- Thermal cyclers for less than $3,000?
WSchick at aol.com
- IGNORE: Test Only
BartFrank
- HELP! T4-RNA Ligase
Chr. Wagenknecht
- HELP
Tisha
- HELP
John Brennand
- Source of TGF-beta1
simon
- (no subject)
ppadula at IMPSAT1.COM.AR
- Help: Alternative to Amersham/USB kit for DNA/RNA isolation
Oivnd Enger
- microsomes
R. Woodward
- subcloning
Michael Witcher
- Help: Alternative to Amersham/USB kit for DNA/RNA isolation
Oivnd Enger
- Long Pcr...Ecperience and Suggestions (2.5 KB from Plasmid + linker)
Graham Dellaire
- ? Counting Cells using a desktop scanner ?
Dennis Templeton
- HELP! IBM-compatible Plasmid Package?
Steve
- Site-directed mutagenesis
Michael Szardenings
- Help: MORPH and Stratagene mutagenesis kits
A.S. Sohal
- G or C at 3' end of primer?
Michael Szardenings
- Plaque hybridisation with nylon membranes?
Carsten Hoege
- Vertical Agarose gels, help
Mr. M.J. Lush
- Northern to Mungo or shiverer
Szabadkai Gyorgy
- Which bacterial species support pUC18/19 replication?
Karel Wernars
- Gedankenexperiment
Michael Benedik
- unwanted plasmid deletions...subhuman
Chris Boyd
- (none)
J. Ibave
- Arabidopsis expression library
Ramesh Raina
- Salmon Sperm & Yeast Xform.
James Smith
- Freezer Storage of Cosmid Library in Microtiter Plates
Michael R. King
- Arabidopsis expression library
Ramesh Raina
- CoT curves? Genome size determination
Susan Jane Hogarth
- Which bacterial species support pUC18/19 replication?
Karel Wernars
- Help:Stratagene and MORPH mutagenesis kits
A.S. Sohal
- Gedankenexperiment
Louis Hom
- (none)
Page
- (none)
A.S. Sohal
- Vertical Agarose gels, help
Robert G. Hamilton
- pegasus
hassane mchaourab
- Anacystis nidulans vs antibiotics
Louis Croisetiere
- Cell cycle analysis by FACS
KELLY THOME
- Help about computers applied to Biology
J. Ibave
- DNA Primers
Dr. Peter Gegenheimer
- Vertical Agarose gels, help
John Brunstein
- DNA Sequencers
Dr. Peter Gegenheimer
- Help: MORPH and Stratagene mutagenesis kits
Dr. Peter Gegenheimer
- Minipreps for ABI sequencing
J. David Spafford
- Wretched dinucleotide repeats
Dr. Peter Gegenheimer
- Single-stranded DNA stability
Karen Nel
- double helix urgently needed
Georg Kroeger
- PCR- Primers Help
Kiran
- SDS & Chelex
Clay Comstock
- RNA Slot-blotting woes
Gabriel Romero
- Phenol Stories
Patrick HJ Falckh
- IL-2 Tissue culture ELISA
Michael J. Herron
- Anacystis nidulans vs antibiotics (fwd)
Edgar Valencia
- Help: plasmid for functional expression
Mark A Osinski
- Minipreps for ABI sequencing
Michelle Gleeson
- Help: Alternative to Amersham/USB kit for DNA/RNA isolation
Oivnd Enger
- Thermal cyclers for less than $3,000?
Tay Hou Ngee Agnes
- Freezer Storage of Cosmid Library in Microtiter Plates
(Dave Johnston)
- Nematode DNA extraction method
Andrew Harrison
- DNA fibre FISH blues
Margaret Leversha
- "Gedankenexperiment"
Denni Schnapp
- PCR Primer annealing temperature calculator - WWW
Jon Boxall
- pCMV-Neo resistance
Graham Atherton
- Protocols for THP-1 transfection required
Daniel Fishman
- quantification of DNA
Els Van Geldre
- HELP HELP cDNA library
Els Van Geldre
- GCK (was "Re: Gel drawing program?")
Josh Goldstein
- Mutation rate study
Hans Stenoien
- rat yac
Rachelle Leger
- To Administrator: Who is Steve OKane?!
Alexander Kraev
- DNA stain
Matthias Hecht
- Ligations - help
Jane McHarg
- biotinylated oligos freeze/thaw?
RMCMAH93 at IRLEARN.UCD.IE
- Genomic DNA Extraction from Tumor Specimens
Margaret A. Keller, Ph.D.
- HELP HELP HELP needed with RNA extraction
c670769 at showme.missouri.edu
- PCR getting smear
c670769 at showme.missouri.edu
- Anyone using p123T-vector?
s060965 at AIX1.UOTTAWA.CA
- Bal 31 information
c670769 at showme.missouri.edu
- HELP HELP cDNA library
KHUANG at CHEMVX.TAMU.EDU
- Have you tried Life Technologies workshops?
Jim Litts
- CNBr Cleavage of soluble proteins
Michael West
- Codon preference usage
Peter Hians
- translocation of PKC?
Maikel van der Velden
- help
hayasihr at kdcnet.ac.jpk
- HA vs myc epitope
Karen Browning
- (none)
Mildad Binas
- vector with ER retention signal????
Thomas Edward Haenel
- Ringer buffer???
Michael Szardenings
- sequence signal
Namy Olivier
- primers for rat
lars.warngard
- Acrylamide Gel setting too quickly
Harry Reis
- Dye terminator mix (ABI) composition?
Pierre Lecocq
- E.coli dies. HELP!!!!!
Kaj Stenberg
- PEG in ligations
nsaunders at molbiol.ox.ac.uk
- anti-TGFbeta antibodies, etc.
Wolfgang Schechinger
- Biotechnology Congress
J. Ibave
- alpha-Lecitine
Holger Bohnemeier
- p53/cell lines
JMARTINEZ at AZCC.ARIZONA.EDU
- Lambda DNA Problems
tera
- Lambda DNA Problems
tera
- 2D PAGE pH gradient
Elke
- (none)
J. Ibave
- Lambda DNA Problems
tjstbc at SD.CTS.COM
- BrdU protocol for In Situs??
Sue Jeffrey
- Why is solubility phe > tyr ?
Achim Recktenwald
- Conc. metabolites in cells ?
Achim Recktenwald
- Problems with sequencing gels
Juergen Serth
- Why is solubility phe > tyr ?
Achim Recktenwald
- Northerns - nitrocellulose or nylon ??
ritaud at rsvs.ulaval.ca
- Anyone have the nucleotide sequence of pcDNA3?
ritaud at rsvs.ulaval.ca
- Lambda DNA Problems
KHUANG at CHEMVX.TAMU.EDU
- MJR cyclers: I want opinions on...
Ed Castro
- (no subject)
tera
- The Biotech BiblioNet
Dyann K. Schmidel
- KGB Buffer-was (none)
Bradley Turner
- MJR cyclers: I want opinions on...
WSchick at aol.com
- RC-5
hassane mchaourab
- PEG in ligations, why?-References
Bradley Turner
- HELP HELP HELP needed with RNA extraction
Thibaud Le Mouel
- pCMV-Neo resistance
Graham Atherton
- Using old slides for FISH
Hans Dauwerse
- Ringer buffer???
Dr. M.R.Barnes
- RNA quantitation
R. Woodward
- Ringer buffer???
Anthony J. Murray
- Re- Old FISH slides
Brett Beitzel
- STRATAGENE'S HybriZAP two-hybrid library kit
Joe Boutell
- cheap IPTG ?
D. Hodges
- Fecal DNA Extraction
Georg Steiner
- Mutagenic primers
Philip Aisen
- DNA Sequencers
Unknown
- primers for IGFBP
David Wilson
- non-radioactive PCR...any suggestions?
ahab
- RT-PCR
NEAL MUSTO
- E.coli dies. HELP!!!!!
Deb Britt
- Colcemid or colchicine cell culture arrest conditions/method?
Herbert Ludewick
- pCDNA3 Cloning Difficulties
Paul Edward Kowalski
- Freeze and Squeeze
Ann M. Yezerski
- prestained SDS-PAGE markers
Soeren G. Gatermann
- Qiagen Web Site?
martin LEACH
- MJR cyclers: I want opinions on
prscholl
- p24 antigen protocol
Mario Hugo Genero
- Heat shocked maize library
dinakar bhattramakki
- BL21(DE3)pLysE
James Smith
- Microinjection of Murine ES cells? References or experiences
Graham Dellaire
- prestained SDS-PAGE markers
Keld Sorensen
- prestained SDS-PAGE markers
Ziping Yang
- Attachment methods fro chromophore onto a protein
M Thompson
- Synthesis method for thiazole orange
M Thompson
- DNAase step in differential display PCR
William Sun
- 2D nightmare
jgluz at STUD.MED.CORNELL.EDU
- 2D nightmare
David C. Logan
- Antibodies against hamster cells
WLC dos-Santos
- Invitrogen's pZero plasmids
Richard Friedman
- Help with Digestion
c670769 at black.missouri.edu
- ExoIII-Why not ApaI?
Markus Piotrowski
- 2D nightmare
David C. Logan
- 2D nightmare
Chi-kuang Wen
- PCR problem
stephane angers
- Rat Type I collagen cDNA probe needed
dginn at olivet.edu
- vector with ER retention signal ?
Thomas Edward Haenel
- Medline and reference manager?
Bjoern Voldborg
- OLIGO 5.0 distributor in Germany?
Henryk Bochmann
- suicidal rna extracter
GERALDINE KAMINSKI
- Isolation of radiolabled RNA
Hilde Nilsen
- Isolation of radiolabled RNA
Hilde Nilsen
- Isolation of radiolabled RNA
Hilde Nilsen
- S: Antibody against Factor IX
schille at uni-muenster.de
- mRNA isolation
Hilde Nilsen
- human epidermal cDNA libraries
Nick Jacobsen
- CSTFA RNA extraction
Szabadkai Gyorgy
- single cell RT-PCR
Szabadkai Gyorgy
- Research Genetics MapPairs PCR primers
john wolff
- nuclear runoff assay
mahayden at med.unc.edu
- Wretched dinucleotide repeats
John Wolff
- Help!!! liagtion problem
Rodney Earl Pettway
- diff display pcr
Elmer Price
- HELP!! Ligation Problem
Rodney Earl Pettway
- Help!!!!!! Ligation
Rodney Earl Pettway
- Problems with sequencing gels
John Wolff
- Phage Display Library
Paul N Hengen
- RNA Slot-blotting woes
Patrick HJ Falckh
- ExpressHyb Hybridisation Solution
Patrick HJ Falckh
- Expression vector selection help
mikel at vaxc.cc.monash.edu.au
- Help!!!!!! Ligation
Amie Franklin
- cDNA labelling
I.S.Wood
- IS-hybridisation: emulsion problem
Mr. M.J. Smith
- Lambda DNA prep. Protocol
John Brennand
- What is Nitro-Block?
Horst Ibelgaufts
- FISH and Banding... What is a good technique (R or G??) ?
Graham Dellaire
- Staurosporin Mechanism
Tim Wachtmann
- HELP!!!!!with protein renaturation (in a soluble form).
Mike Faughn
- HELP HELP HELP needed with RNA extraction
/G=Rimantas/S=Plaipa/OU=GF/O=VU/PRMD=LITNET/ADMD=LITPAK/C=LT/ at mhs-relay.ac.uk
- Brain Nuclear Extracts
Sandra Pena De Ortiz
- CASTING METAPHOR AGAROSE GELS
Perry J. Bushong
- Phage-Display Libraries.
Lakmal Boteju
- Promega dual luciferase system
jfh
- PCR-What is in my reaction?
Perry J. Bushong
- CASTING METAPHOR AGAROSE GELS
John Wolff
- TKO Fluorometer
John Wolff
- Primer Design
Dr. Philip Aisen
- Phage Display Library
RVDESHP0 at am.pnu.com
- Animal Pharm Services
billing at vetsci.microvet.arizona.edu
- genome size/complexity
Susan Jane Hogarth
- BRP vector (pSW1 only) sequence or restriction maps.
David B. Knaebel
- PCNA westerns
Brian McCauley
- Help with ligations
Henry Wong
- Brain Nuclear Extracts
martin LEACH
- Promega dual luciferase system
martin LEACH
- How to break out bacteria on the plate
Jung Hoon Ahn
- Help! Seeking Moermann Therapy
Paul A. Kowal
- diff display pcr
Smith Group
- How to break out bacteria on the plate?
Jung Hoon Ahn
- Generation of microsatelites from clones
G.P.
- Rep : Medline and reference manager?
Mr SHIRE David
- mRNA abundances
Dr. T.F. King
- How would you define a "cloning artifact"?
Horst Ibelgaufts
- BRP vector (pSW1 only) sequence or restriction maps.
DAVID B. KNAEBEL
- CASTING METAPHOR AGAROSE GELS
STEVEN GERHARDT
- G-free vector p(C2AT)19
Abraham Rosenberg
- Semi-dry blotter recommendations
Louis Hom
- Anyone successful with Pharmacia RPAS?
Deb Britt
- Lambda DNA prep
Chi-kuang Wen
- Shareware and Freeware for Science
Catherine O'Malley
- Glutathion in Bacterial Media
corboy at utdallas.edu
- Shareware and Freeware for Science
corboy at utdallas.edu
- pBR322 tet Antibody
Scott Taylor
- Shareware or Freeware for Science
Catherine O'Malley
- pRSET/histag expression problems
Drobert2 at utk.edu
- Q: Best method for precipitation of small RNA's ( 10-20nt ) ?
User
- Glutathion in Bacterial Media
corboy at utdallas.edu
- lipid IVA
felix randow
- NF-kappa-b
felix randow
- strider program
RUTH MCMAHON
- cos-cells and nf-kappa-b
felix randow
- DNA Extractions
Drew Avery
- daemons!
martin LEACH
- the simple fools guide to PCR
Alice Hempel
- Primer checking website?
Bill Burnett
- RNAses: Fact over Fable!
James Graham
- DNAse at pH5?
James Graham
- daemons!
Edgar Valencia
- sequencing gel won't stick to Whatman paper
jlight at SICKKIDS.ON.CA
- The %"%#@ bounced message
Edgar Valencia
- HELP!!!!!with protein renaturation (in a soluble form).
Scott McMahan
- sequencing gel won't stick to Whatman paper
(Dave Johnston)
- Long biotinylated DNA binding to magnetic particles
piero carninci
- Isolation of "hot" oligos
Joachim Kremerskothen
- Isolation of "hot" oligos
Francois Coulier
- pG+host Vector.
M.C. Fontaine
- A260 absorption of free nucleotides
R. Woodward
- Seeking source of DEFENSIN antibodies
Bradley Turner
- RT-PCR FROM **SMALL** BIOPSIES
P.G.Mcculloch at LIVERPOOL.AC.UK
- A260 of free dNTPs
nsaunders at molbiol.ox.ac.uk
- Wilms' Tumour(tumor) cell lines
Mr. D.M. Shaw
- NIH 3T3 Cells
Marcel Hoffmann
- NIH 3T3 Cells
Curt Ashendel
- pswy1
David B. Knaebel
- Unbreakable bugs!!!
Philippe Lefebvre
- Very high background!!! Help!
Dr. L. Darjania
- Help! Protocol for electroporation of V. faba guard cell protoplats.
Dr. L. Darjania
- 5' RNA processing - ribozyme
Michael Goodin
- Unbreakable bugs!!!
Randall Willis
- Can I pre-stain Prot markers for W. Blot?
Dave
- DIG vs. biotin: Preferencess?
John J Weiland
- PCR primers and clones for human genes
Amy Voltz
- Can I pre-stain Prot markers for W. Blot?
Ian A. York
- lipid sources
rhmorse at top.monad.net
- PCR-dilema!
paola velasco
- EBNA1 positive cell line
Matthias Vogel
- daemons!
H. ROYCHOWDHURY
- who sell methicillin
X Jia
- Can I pre-stain Prot markers for W. Blot?
Dave Neece
- Help need different CAT plasmid
TankerTom
- Additives and PCR Fidelity
vilimf01 at mcrcr6.med.nyu.edu
- DNA extraction from urine
gturbett at eosin.path.uwa.edu.au
- Immunodiagnostic Distributor Wanted
hreynolds at uh.edu
- Fast purification of protein from DNA
nobody at nowhere
- DNA extraction from urine
Dave Neece
- Restriction digests
Przemko
- Please, help with RNA extraction.
melia at ar.vhebron.es
- Nissim scFv phage display library
simone at erdw.ethz.ch
- ANNOUNCEMENT: Understanding Protein Structure Determination
Lennart Nilsson, KI/CSB
- Re-use of electroporator cuvettes
M.C. Fontaine
- Inoue again....
Paul
- Help Northern Blocking!
Mark Watson
- promoters controlling truncated mRNAs
Luc Ferrari
- Please, help with RNA extraction.
John Brennand
- Help Northern Blocking!
John Brennand
- cDNA library
chun taehoon
- A-20 Electroporation Protocol
George W Chacko
- Blunt-end cloning
Chi-kuang Wen
- RT-PCR vs. RNase protection assay
Shelley Cole
- Gibco BRL "Genetrapper" screening method
J. David Spafford
- IGF-I, Non-Extraction
hreynolds at uh.edu
- Osteoporosis-Bone Resorption Marker
hreynolds at uh.edu
- pQE32 expression vector sequence
l
- GroEL concentration
Geri Grant
- PCR primers for IGFBP-1
David Wilson
- anti-TGFbeta antibodies, etc.
Jorge Garrido
- Blunt-end cloning
John Donald
- test
lisa Liu
- test
lisa Liu
- test
lisa Liu
- test
h-liu1 at uiuc.edu
- Spi selection strains
Sally Ann Adler
- About *TEST*
Edgar Valencia
- hints wanted for running formamide sequencing gels
jlight at SICKKIDS.ON.CA
- DyNAQuant 200
William Naughton
- HELP ! get DNA (< 500bp) out of gel
Hong Lin
- HELP ! get DNA (< 500bp) out of gel
Nick Jacobsen
- DNA-editing under Windows
Markus Piotrowski
- Snap Freezing: N2 or Isopentane?
Kimberly Martin
- problem cloning a PCR amp. fragment
akhan at usa.pipeline.com
- Q:cell cycle analysis on living cells
ronlab
- Q.?? Are there culture dishes with grid lines for counting cells?????HELP
Ivan Brukner
- Counting tranfected cells carrying betagal? Ideas and suggestions?
Ivan Brukner
- BetaGal... Counting cells?? Suggestions ?
popa0206 at PO-BOX.MCGILL.CA
- I need some advice on bandshift assays
Tom Magee
- HELP ! get DNA (< 500bp) out of gel
Chi-Kuang Wen
- NaI solution in geneclean
jie li
- Ladder Help
Clay Comstock
- cDNA sequence differences
Leo
- (none)
Dr. Min Peng
- cDNA sequence differences
Nikolai Chitaev
- Cosmid end sequencing
Jeffrey Keen
- psw1
Dave Knaebel
- Intracellular dNTPs and rNTPs concentration
Durochd at IRCM.UMontreal.CA
- MJR cyclers
christopher chow
- Needed: pCMV sequence
Chia Jin Ngee
- daemon
Denni Schnapp
- Dot-blot automation
Peter Kuhnert
- Differential Display-Clontech, Display Syst. or GenHunter Help.
Mary P. Remington
- Restriction fragment mapping
J. Pat Martinez
- T7 transcription of oligos
Paul Digard
- Anti-phosphothreonine in ELISA (KIRA assay)
Kevin Moulder
- Pellet Paint/DNA Paint
Keith L. Ligon
- QuikChange=QuickWaste?
Keith L. Ligon
- MW of PVP inDenhardts?
Greg Galloway
- HELP -- pcr mystery
Juliewoods
- cDNA sequence differences
Thomas C. Newman
- Low-Bis PAGE
D. KIM
- AMINO ACID ASSAY
Stewart, Robert
- Kodak CD40
Soren K. Rasmussen
- Cheap wheat germ lysate?
Scott Vande Pol
- Pellet paint/DNA Paint
KHUANG at CHEMVX.TAMU.EDU
- Contig Analysis software
Don Wigington
- DNA / RNA dipsticks
kanemats at morgan.angis.su.oz.au‚
- testing
COco mAN
- formaldehyde gel for RNA
Wong Yong Wee
- Question about gel shift assay
SAI-Jinling Xie
- Ribonuclease Protection Assays
jon
- Cheap wheat germ lysate?
Amie Franklin
- Long PCR suggestion!
KHUANG at CHEMVX.TAMU.EDU
- In vitro transcription kits & other RNA reagents
Donald Chun Kit Wong
- MW of PVP inDenhardts?
Thibaud Le Mouel
- re mw of pvp in denhardts
Thibaud Le Mouel
- Please, help with RNA extraction.
Thibaud Le Mouel
- deletion mutant in Cyanobacteria
Farid jalali
- Silver Staining Protocol
Michael Daws
- Free energy of hairpins and their Tm
A.P.R.M. OSTEROP at TCH
- mRNA for cDNA library
Els Van Geldre
- Best Instrument for PCR?
WSchick at aol.com
- Begining with Baculovirus
jpl at ibcp.fr
- protein kinase activity
delpierr at univ-lille1.fr
- protein elution
J Burger
- HELP!!!!!with protein renaturation (in a soluble form).
QIAGEN
- (none)
A.S. Sohal
- Silver Staining Protocol
Dima Klenchin
- slicing acrylamide gels
Guido Hooiveld
- GenHunter
Jill Kellogg
- DNA/RNA dipsticks
Jill Kellogg
- problem cloning a PCR amp. fragment
Jill Kellogg
- pcr cycle
Shi-Jye Chu
- RNA extraction with 5.2S Band
lcwilliams at MEDUSA.UNM.EDU
- pcr cycle
Andrei Popov
- pcDNA2 sequence
Jean-Francois Larrivee
- Best Instrument for PCR?
Michael Casey
- AMINO ACID ASSAY
Phil Robinson
- Rat cDNA library query
timmer at physio.emory.edu
- Gene targeting vectors - recent reviews?
Ashok Aiyar
- polytron for RNA extraction
Dianne Emslie
- nuclear run-on for low abundant RNA
Mari L. Shinohara
- intensifying screens
Robyn Wallace
- check method of cell lysis
Jung Hoon Ahn
- pcr cycle
Thibaud Le Mouel
- intensifying screens
(Dave Johnston)
- Error rate of Reverse Transkriptases in cDNA synthesis
Karl Schmid
- Antibodies against hamster cells
WLC dos-Santos
- Gene targeting vectors
Chris Paszty
- epitope tagging in plants
Alfredo Lopez De Leon
- Bacterial cDNA
Alfredo Lopez De Leon
- Intensifying screens
Paul Digard
- pcr cycle
nsaunders at molbiol.ox.ac.uk
- Molec Bio Method Book
Anusak Pinyopich
- Intensifying screens
Laurence Tiley
- TELOMERASE IN CULTURE
Steve Griffiths
- epitope tagging in plants
STLUND at GNV.IFAS.UFL.EDU
- non-radioactive sequencing
Carl Raimond
- Intensifying screens
S.K.Green
- sequence of lambda YES and/or gt6
Helen Logan
- RNA/DNA isolation
Joe Maa
- Photodocumentation?
sang at tapioca.bf.epm.br
- MORPH mutagenesis kit
A.S. Sohal
- Pointer to FAQ lists
Paul N Hengen
- IMIDAZOLE BUFFER
Osvaldo Marinotti
- intensifying screens
H. ROYCHOWDHURY
- Did someone use 5'RACE kit from GIBCO/BRL?
Cheng Luo
- Semi-automated sequencing w/ NIH Image
Tige Broadhead
- Why are concentrated enzymes more stable?
Tracy Aquilla
- Luminometer
Ramesh Raina
- question about virus infection (MOI)
Ying Wu
- Sequencing from chromosomal DNA
Rafael Maldonado
- polytron for RNA extraction
Dianne Emslie
- intensifying screens
Pamela Norton
- Replication Origins and Two D gels... Question??
Graham Dellaire
- Acetyl transferase... has anyone found a mammalian homolog??? to gnd5p?
Graham Dellaire
- Luminometer
WSchick at aol.com
- Need of Ultracentrifuge rotor
Kishor Wasan
- JetSorb
heme
- (none)
Ryu Chun-Jeih
- test
Jeih
- Difficulties with PCR of alfa-globin gene!
Nuno Miguel Ferrand
- jolly good
Netscape Student
- agrobacteria and/or Ti plasmid newsgroup???
Jeff Velten
- cell energy metabolism and HPLC
Andreas Jordan
- protein/peptide elution from nitrocellulose/PVDF
Dennis Takayesu
- Quantifying DNA
Lisa M Miller
- ribosome 3-dimensional structure?
Stephan Witte
- Problems in a fish genomic library construction
LUIS BEJARANO ARDURA
- cDNA through mail
Barclay Morrison III
- create artificial membrane
Ying Wu
- PCR specificity with cDNA library as template
SAI-Jinling Xie
- Solubilizing bisacrylamide-crosslinked polyacrylamide gels
Mike Perry
- Advice on Miniprep Machine?
Jac A. Nickoloff
- Help on PCR
bartoli marc
- purification of GST fusion proteins in presence of detergents
jlight at SICKKIDS.ON.CA
- [Q] ThermoSequenase aging
Eric Kofoid
- French Press
Philip Heacock
- suppliers of transilluminator parts
KrisPrasad
- lipid IVA, compound 406
felix randow
- TNFRs in squamous cells
mir00014 at WARI.RCP.NET.PE
- electroporators
Linkman
- Rifampicin activity
Wes Hatfield
- Dextran sulfate: replaced by PEG?
Belhumeur Pierre
- Help! RNA Protection Assay
Anton Tutter
- DNA in ELISA?
Michael Adamson
- Quantifying DNA
JorisVERLOOYverlooy at uia.ua.ac.be
- Subtractive cDNA
MARK PEGGIE
- Advice on cDNA selection
Nick Jacobsen
- Books?
Ian Brandt
- extra bands in SSCP'S
Ian Howell
- thawing & plating cell lines
cram at PIPELINE.COM
- giant taro (alocasia macrorrhiza) seeds or DNA .. HELP!
Massimo Delledonne
- Brassica campestris cv. pekinensis
Massimo Delledonne
- Looking for E. coli TOPP6
Fran=?iso-8859-1?Q?=E7ois Carrel
- quick ligation?
Alan Hair
- ligand
glycotech
- Glucose-1-phosphate levels
Jose M. Bautista
- Beginner with RNA extraction
Edouard Lauzier
- oligos -> gene
Tom Doak
- staining of living cells
Fritz Schwarzmann
- Azidopine binding and pgp
Geri Grant
- a good PCR mimic?
Bert Grobben
- How do I label my PCR probe?
Jackie Song
- thawing & plating cell lines
martin LEACH
- Linecott's Guide
S. Kohler
- x-gal plates for yeast
Przemko
- RE- Linscott's guide
Kathleen Liu
- Acridine Orange
Clay Comstock
- Antibodies to Dansyl Group
pwpark
- Antibodies to Dansyl Group
Robert Mecham
- thawing & plating cell lines
Andreas Jordan
- 293 Cells: how to transfect?
iddavis at vms.cis.pitt.edu
- PCR artifact?
Klaus Salger
- 293 Cells: how to transfect?
jstrassw at opal.tufts.edu
- antibodies to yeast proteins
DSF
- Yeast Transformation
Liz Horton
- Synperonic (PE/F68)
Tom Parish
- Differential Display
Grete Froerup
- 4 way ligations?
Angel Yanagihara
- Competent Sure E.coli?
TJ Murphy
- cycle sequencing from agarose
varyc.mmcwp4 at mmc.org
- pME18S
Kathleen Liu
- (none)
postmast at iihema.fmed.uba.ar
- DNA-Elution from gel
Oliver Berkowitz
- DNA-Elution
Oliver Berkowitz
- 4 way ligations?
Ned Mantei
- More question about gel-shift assay
Leo
- Highlighting Sequence Alignment
Stu Johnston
- double protein expression
Karen Browning
- Fluorescent Oligo's
Jeffrey Upperman
- test
yu
- Monoclonal antibody against GST fusion protein
yu
- Info needed on Scientific dictionary
dinakar bhattramakki
- Use Gemini serum and channel blockers?
GidgetCat
- DNA on Glass or Plastic
Stu Johnston
- Rep : a good PCR mimic?
Mr SHIRE David
- cloned mucins
Gustavo Nolasco
- AGAROSE BEADS
macnetcw at wave.co.nz
- Comet assay
JS Harvey
- Frequency of BstE II cutting?
W R BENNETT
- Western with sheep-IgG's
room 103
- Sheep IgG's with Email
room 103
- 2D-PAGE and Clostridium
Michael Kertesz
- [Q] ThermoSequenase aging
Eric Kofoid
- slicing acrylamide gels
Eric Kofoid
- Liposomes for ion transport - questions, questions, questions
Mike Holloway
- XL-1 Red mutator strain
Danny Altschuler
- mutagenesis of a huge gene
benan dincturk
- thawing & plating cell lines
Curt Ashendel
- GST Fusion protein
TREACYB at NCCCOT.AGR.CA
- long oligonucleotides
The Mighty Shing
- Nuclear extracts from peripheral blood lymphocytes
Chris Hughes
- Cloning PCR product
Helen McBride
- Did airport security destroy my DNA?
Cathy Sprankle
- enzyme kinetics programs??
Robert Baker
- Critical decision!!!
Jurgen Vanhauwe
- y
Grete Froerup
- y not
John Ladasky
- test
hreynolds at bayou.uh.edu
- cyanobacterial transformation
Farid jalali
- Help! chloroplast isolation
Zhu Congju
- Problems with Hybaid Omnigene Thermal Cyclers
Jill Maddox
- [33P]-alpha-ATP
Tim Young
- pGEX Vectors
Simon Dawson
- RT-PCR-Multiple bands-unusual problem
Paul Mozdziak
- Problems with Hybaid Omnigene Thermal Cyclers
Ashok Aiyar
- TRANSPOSASE SOURCE
Chromosome Terror
- nuclear extracts/footprinting
Bruce C. Kone, M.D.
- BRL Random Priming
George Rutherford
- LIC Vector Kits from Novagen
Dr N. Gormley
- test message
David D Morgan
- Kodak film gone yellow
RUTH MCMAHON
- ThermoSequenase
David D Morgan
- RNA secondary structure predictions - G-U pairing
reset
- 3' RACE Difficulties
Nanto Cielens
- PCR primers for rat IFGBP-1
David Wilson
- 2',3'-cUMP Info.
Nanto Cielens
- Help find K cacodylate source! Thanks!
Robert Cowherd
- need peptide sequencing service by MS-MS
Yi-Quan Tang
- Cleanup of short PCR Product
Michael J. McLeish
- HELP! VENT / Pfu amplification
Obaid Y. Khan
- Kodak film gone yellow
Rimantas.Plaipa at GF.VU.lt
- Rubidium?
petros at wam.umd.edu
- alpha-thio-ribo nucleotide triphosphates
matt thomas
- microsatellite development
Diana Wolf
- AntiFlag polyclonal antibodies
Joe Ecker
- Agrobact PCR
Jeff Velten
- HELP! VENT / Pfu amplification
C.Pical
- airport security
Denni Schnapp
- Antibodies against cytoskeletal proteins & molecular motors
Thomas R. Anderson
- Human smooth muscle cell culture.
Pippa Deex
- Separation of bacteria by hydrophobic interactions
Jeremy Carson
- Chromosomal localisation
DOCON at ollamh.ucd.ie
- Commercial source of Charon Lambda?
John Dixon
- cDNA library
Corinna. Dutton
- inosine in PCR primers
Martin Goodson
- RNA Extraction... ( Still )
Edouard Lauzier
- RNA extraction using ULTRASPEC RNA from asthmatic monocytes.
Binnie Betten
- Ligating large fragment into vector
jimmy barbee
- eukaryotic heterochromatin-specific probe
Stephanie Tucker
- IMPORTANT - news server problems at net.bio.net
BIOSCI Administrator
- Amoeba proteus transfection?
Michael J. Herron
- RNA Question
Edouard Lauzier
- Isolation of Genomic DNA from Starfish
Ray Colliton
- Question re oligo synthesizers
Allison Haggarty
- in vitro transcription
Yiming Bao
- E.Coli Lac deletion, Nal -s strain
HelenCS
- Co transafection efficiences-liopsome or Ca Po4
Stu Johnston
- HELP! VENT/ Taq polymerase
Mail at scri.sari.ac.uk
- Amoeba proteus transfection?
Dima Klenchin
- Commercial source of Charon Lambda?
Peter Wang
- Yeast expression vectors !!!!!!!
Pathology
- Rep (2) : a good PCR mimic?
Mr SHIRE David
- NgCAM; TAG-1 & L1 (NILE) cDNAs
Dr. P.A. de Zoysa
- DIG Northerns
Graham Atherton
- Primer storage
Kenneth Kao
- Ligating large fragment into vector
Graham Dellaire
- ?Measurement of ATP
Andrew Williams
- Templates for ABI Genescan
:
- Antibiotic Potency Assays
Rosemarie Lieffring
- DIG Northerns
Klaus Salger
- Protein expression
Karen Nel
- Ready-to-go cleaning for e-poration
Lars Janzon
- HB101 media for Hybridomas
Michael_J_Hansbury
- Protooncogenes expression- cDNA versus RNA Probes
negar moshiri
- GFP
Douglas Prasher
- Rep (2) : a good PCR mimic?
Richard Pelletier
- DIG Northerns
Jean-Francois Larrivee
- culturing microbes
clee at physics.ubc.ca
- Test
Merce
- histone deacetylase inhibitors
Douglas V. Faller
- Swiss 3T3 transfection
Didier Thomas
- blue colonies-no Xgal
Michael H. Bracey
- RNA Extraction... ( Still )
Thibaud Le Mouel
- DIG Northerns
Thibaud Le Mouel
- weird RNA pattern
Wong Yong Wee
- Lactose instead of IPTG
Fiona Thornton
- Monoclonal Antibody databases
Bruce Freeman
- Lipidex-1000; cholesterol binding assay
simon
- !!!!!! Antibody against tissue factor !!!!!!
P. Friedl
- Transformation reagent
Paul D. Thompson
- Transformation reagent
Paul D. Thompson
- Post-ligation restriction
Jean-Paul CADORET, Gie-ra Montpellier PDG-DRV-RA-DRIM, +33-6714-4707
- Lactose instead of IPTG
Martin Rink
- Recipe for citrate-phosphate buffer?
Namjin Chung
- poly A+ purification
Szabadkai Gyorgy
- DNA-binding resins
Peter Chen
- DNA-binding resins
Ashok Aiyar
- Enterokinase
WUSC
- Ligation Independent Cloning
Rockey Kumar Bandlish
- problems with background doing in vitro transcription/translation
jlight at SICKKIDS.ON.CA
- cloned mucins
Bradley Turner
- Are there any B1 repetitive mouse probes out there?
Karina McQueen
- in situs with oligos?
Majumder lab
- in situs with oligos?
Majumder lab
- dem bones
ImmGen Inc.
- Cryophile Polymerases
Brett Beitzel
- Web site for blood product reagents
slweho
- weird RNA pattern
Bernard Murray
- triphenyltetrazolium chloride indicator agar
Toai T. Nguyen
- GibcoBRL/Lifetechnologies
Cheng Luo
- DNA elution from gels
Mr. Chris Hoyle
- test test
SNAKE15086
- RNASE DECONTAMINATION REVIEW
SNAKE15086
- ho ho
SNAKE15086
- Mutagenesis
Robert Mecham
- Cleanup of short PCR Product
LeeSylvers
- Teacher seeking used equipment
Davidr222
- Source for Aminoallyl-dUTP
Jens Alfken
- ? about DNA extraction kits
Juliewoods
- Tm calculation short and long oligo's
Niels Groot
- wierd RNA pattern
Rosemary T. Hoffman
- HMGCoA Reductase
Christine Gee -BIOCHEM
Last message date:
Sun Jun 30 21:27:33 EST 1996
Archived on: Sun Mar 6 21:06:41 EST 2005
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