Density gradient cell separation and streptavidin-agarose?

G.P. goran at atax.neur.uni-duesseldorf.de
Fri Mar 1 02:27:49 EST 1996


I am trying to isolate cells from primary culture which bind 
streptavidin but I do not have ability to use magnetic beads.
So I thought to use streptavidin-agarose instead and try to separate
the rest of the cells from streptavidin-agarose coupled ones by
using density gradient like phycol or similar things.

Any experience with a system like this out there or any suggestions?

Goran

goran at atax.neur.uni-duesseldorf.de



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