Making His-tag affinity resin

Rita Lim lim at bscr.uga.edu
Fri Mar 1 16:42:44 EST 1996


Hello to all protein chemistry scientists out there.
I like to know if anyone has ever made their own histidine affinity resin
for the
purification of histidine-tag recombinant proteins.
Or is it more convenient to buy the resin from the various companies that
sell them.
Suggestions and comments are welcome. Thanks!
Rita



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