Sequencing after G run

Michael Gorry mcgorry at med.pitt.edu
Fri Mar 1 15:31:15 EST 1996


I am having a problem sequencing a clone.  Using the taq-FS
dyedeoxy terminal cycle sequencing kit, I have reached a point
with 21 G's and get 1 more base call before the reaction quits.
A manual sequencing kit was also used with the same results.
Does anyone have any advice on how to get through this stop?

Thankyou



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