***Multiplex PCR Hell******

negar moshiri negar at wam.umd.edu
Fri Mar 1 10:23:19 EST 1996


We recently set up a multiplex PCR in our lab. Results showed 
specific and clear amplification. Without having changed the basic 
protocol, the rxn is now giving us non-specific amplification along 
with intense smearing. Subsequently, we have tried to optimize the 
cycle, Mg++, and Primer parameters but with very little improvement. 

We are going to try decreasing the taq and dNTP concentrations. Are 
there any other suggestions?



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