***Multiplex PCR Hell******
negar at wam.umd.edu
Fri Mar 1 10:23:19 EST 1996
We recently set up a multiplex PCR in our lab. Results showed
specific and clear amplification. Without having changed the basic
protocol, the rxn is now giving us non-specific amplification along
with intense smearing. Subsequently, we have tried to optimize the
cycle, Mg++, and Primer parameters but with very little improvement.
We are going to try decreasing the taq and dNTP concentrations. Are
there any other suggestions?
More information about the Methods