Magnetic RNA isolation

M.W. SMITH BMB6MWS at leeds.ac.uk
Fri Mar 1 11:56:31 EST 1996


Can anybody comment on their experiences with mRNA isolation using 
magnetic beads e.g. from Promega or Dynal. I need to make high quality 
poly a+ RNA for construction of a cDNA library. These manufacturers claim 
that their kits can be used for direct isolation of poly A+ from tissue, 
without the need to prepare total RNA. Does this provide high quality 
poly a+? Any comments would be gratefully received.
Thanks.
Mark Smith (m.w.smith at leeds.ac.uk)
University of Leeds.
UK.



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