PCR probes by incorporation of 32P ?

Geoffrey Neale geoffrey.neale at STJUDE.ORG
Fri Mar 1 11:18:09 EST 1996


Hello everyone.

I am looking for a method for making 32-P labeled probes during PCR. The
information that I specifically need is the concentration of cold 
nucleotide in the reaction so that you generate sufficient full length 
probes, but still retain high specificity of the isotope label.

Other considerations are the amount of starting template, the 
concentration of primers, and the number of cycles.

Thanks for your input.

Geoff Neale

PS Could you please email me your advice since our news reader is not yet 
operational on our system. E-mail is my only access at this time. Thanks.

geoffrey.neale at stjude.org



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