SfiI enzyme

Andrei Popov ANDREI.POPOV at bbsrc.ac.uk
Sat Mar 2 08:10:45 EST 1996


>Has anyone had difficulties ligating PCR products following Sfi1
>digestion?  We are using Geneclean to gel purify PCR products following
>digestion with various restriction enzymes.  All products seem to ligate
>efficiently with the exception of those cut with Sfi1.
 
Hi
I suggest to have a close look at the SfiI site sequence
ggccNNNNNggcc
Unless you have indentical NNNNN in  your primers and THE vector
it will not fit. 
regards
Andrei






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