Sfi 1 enzyme
Jeff 'Newt' Sekelsky
fznewt at peseta.ucdavis.edu
Sat Mar 2 12:18:57 EST 1996
On 1 Mar 1996, tera wrote:
> Has anyone had difficulties ligating PCR products following Sfi1
> digestion? We are using Geneclean to gel purify PCR products following
> digestion with various restriction enzymes. All products seem to ligate
> efficiently with the exception of those cut with Sfi1.
Are you sure the ends are comaptible? The SfiI recognition sequence is:
5' G G C C N N N N N G G C C 3'
C C G G N N N N N C C G G
If the ends you're trying to ligate are indeed compatible, then I'm not
much help to you.
More information about the Methods