EtOH + PBS = precipitation?
hutchins at fiona.umsmed.edu
Sun Mar 3 10:44:17 EST 1996
LU Pin (bolupin at uxmail.ust.hk) wrote:
: Recently when I tried to prepare 95% ethanol in PBS, I always got the
: same problem. When PBS was added into abs. ethanol, the solution became
: turbid. Later, some white precipitate formed. Shaking or vortexing during
: PBS addition did not help. The PBS I used contains NaCl, KCl, Na2HPO4,
: KH2PO4. Anyone knows what's wrong or how to avoid this? Thank you very much.
Why are you trying to prepare 95% EtOH/PBS? As far as I'm aware, phosphate
precipitates under the conditions described.
If you tell us the intended use, maybe we can suggest a substitute.
Assoc Prof Anatomy, Asst Prof Neurology (Research), Univ Mississippi Med Ctr
http://fiona.umsmed.edu/~hutchins/ *** E-mail: hutchins at umsmed.edu
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