gillinm at agric.nsw.gov.au
Sun Mar 3 17:58:45 EST 1996
I have used a method from low melt agarose that we used to call the
"Freeze-squeeze". Cut your band from the agarose and place it into a
folded piece of parafilm in the freezer. When it is completely frozen
place the agarose parafilm sandwich between two sterile microscope
slides. Make sure that some of the parafilm sticks out the sides of the
setup. Form a "V" with the microscope slides that is in the same
orientation as the "V" of the parafilm. Slowly squeeze the slides
together, and as the slice defrosts under the pressure, the buffer and
some of the DNA will come out of the agarose. Collect the droplet of
buffer from the parafilm fold.
This works best for small molecular weight fragments (naturally). Phenol
extraction will remove the bromophenol blue.
Dr Michael Gillings
Senior Research Scientist
Biological and Chemical Research Institute
PMB 10, Post Office Rydalmere
On Thu, 29 Feb 1996 vsood at acs.ucalgary.ca wrote:
> I remember seeing some advice on retrieving DNA from agarose slices via a
> method of freezing the slices and then mashing them, but don't recall the
> details. Does anyone use this method who can refresh my memory? Also, what
> about any EtBr or bromophenol blue (my band runs at the same place as the
> dye front) present in the slice?
> thanks a lot.
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