Transfection of proteins into cells: HOW?

brett brett at BORCIM.WUSTL.EDU
Mon Mar 4 20:15:15 EST 1996


> 
>I want to block proteins in cells with specific antibodies,
>and I'm looking for possible methods to get this antibodies
>into these cells.
>Problems:
> 
>- microinjection is not possible because I work with suspension cells
>  (hard to catch and even harder to find the injected ones to check
>  the result)
> 
>- electroporation is *not* the way to (please don't ask why. The story
>  is to long to be told here I think).
> 
>What other methods are possible to get antibodies or other proteins into
>suspension cells?
> 
>Thanks in advance
> 
>Stefan
> 
> 
> 
> 
>--
>Stefan Kahlert, Medizinische Universitaets-Poliklinik Bonn
>UZS13B at ibm.rhrz.uni-bonn.de or UZS13B at dbnrhrz1.bitnet

You can get T7RNAP into cells using liposomes 


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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