Amersham Hybond N problem

Tori twilliam at BIOMED.MED.YALE.EDU
Tue Mar 5 15:42:05 EST 1996


I also used the Hybond-N recently and had a horrible time. According to
the et br staining I had run the perfect gel with genomic dna but after
transfering and probing and exposing I had a solid black rectangle. I
called the company and they said that I need to soak the membrane in 5x
SSC before probing. They also gave me some suggestions for stripping the
filter but nothing worked. I was using unpurified 32P random
hexanucleotide labeled DNA fragments. I soaked the membrane the second
time around and reprobed but all I got was high background and
hybridization to my mol wt markers. I am going to try to reprobe and see
what happens. 

***************************************************************************
                             Tori D. Williams
                       twilliam at biomed.med.yale.edu

     DST SK DK PHD2B SCI 0(+> LIVELONG... AGGIEPRIDE MAC  = ME!!!! :)
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On Fri, 1 Mar 1996, Paul N Hengen wrote:

> BRITTA SCHULZ (schulzb at pz350.ipsp.uni-hohenheim.de) wrote:
> 
> : Who can help me to find out, which company produces the uncharged membrane
> : formerly sold by Amersham as Hybond N ?
> 



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