PMSF timing

Marc Visconti marcvis at resunix.ri.sickkids.on.ca
Tue Mar 5 10:40:22 EST 1996


How are you preparing PMSF?  You should call Sigma and get the specs 
on it, but PMSF works best when solubilized in anhydrous ethanol and 
added to buffers dropwise with slight agitation.  I add PMSF prior 
to cell disruption and have no problems with degradation.

marc



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