Why use 3´to5´exo-negative DNA-Polymerases?

Horst Ibelgaufts Ibelgauf at lmb.uni-muenchen.de
Tue Mar 5 04:51:43 EST 1996

Dear fellow netters, maybe it is a stupid question, but I have been
wondering about the rationale behind using DNA polymerases specifically
designed to be devoid of a 3´ to 5´ exonuclease activity (such as
Sequenase or Taquenase). Is it that these enzymes have a higher
processivity and polymerize faster?

thanks for any enlightenment :-)

and greetings from sunny bright (but cold) Munich



                                                   H IBELGAUFTS
                                     Ultra posse nemo obligatur

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