Transfection of proteins into cells: HOW?

elizabeth Ferguson eaferg00 at pop.uky.edu
Tue Mar 5 09:35:55 EST 1996


UZS13B at ibm.rhrz.uni-bonn.de (Stefan Kahlert) wrote:

> 
>I want to block proteins in cells with specific antibodies,
>and I'm looking for possible methods to get this antibodies
>into these cells.
>Problems:
> 
>- microinjection is not possible because I work with suspension cells
>  (hard to catch and even harder to find the injected ones to check
>  the result)
> 
>- electroporation is *not* the way to (please don't ask why. The story
>  is to long to be told here I think).
> 
>What other methods are possible to get antibodies or other proteins into
>suspension cells?
> 
>Thanks in advance
> 
>Stefan
> 
 
> 
>--
>Stefan Kahlert, Medizinische Universitaets-Poliklinik Bonn
>UZS13B at ibm.rhrz.uni-bonn.de or UZS13B at dbnrhrz1.bitnet


Have you thought about liposomes?




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