Re- Touchdown PCR Protocol
brett_beitzel at MSMTP.IDDE.SACI.ORG
Wed Mar 6 09:42:34 EST 1996
Subject:Re: Touchdown PCR Protocol Date: 3/6/96
I usually start my touchdown PCR about 5 degrees higher than the maximum
predicted annealing temp. For example, if you are using 63C now, try 68x3,
67x3, 66x3, 65x3, 64x3, 63x25.
You can also try throwing in DMSO to increase specificity. Up to 10% DMSO
won't inhibit Taq polymerase.
brett_beitzel at msmtp.idde.saci.org
Cancer Therapy and Research Center
San Antonio, TX
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