Re- Touchdown PCR Protocol

Brett Beitzel brett_beitzel at MSMTP.IDDE.SACI.ORG
Wed Mar 6 09:42:34 EST 1996


Subject:Re: Touchdown PCR Protocol                 Date: 3/6/96

I usually start my touchdown PCR about 5 degrees higher than the maximum
predicted annealing temp.  For example, if you are using 63C now, try 68x3,
67x3, 66x3, 65x3, 64x3, 63x25. 
You can also try throwing in DMSO to increase specificity.  Up to 10% DMSO
won't inhibit Taq polymerase.

Good Luck,
Brett Beitzel
brett_beitzel at msmtp.idde.saci.org
Cancer Therapy and Research Center
San Antonio, TX




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