rkoedood at bio.bu.edu
Wed Mar 6 16:16:36 EST 1996
As far as I can remember, COS cells are C127(?) cells transformed with SV40
large T Antigen, COS7 has more copies of T Antigen than COS1. Therefore,
expression vectors carrying the SV40 origin of replication should replicate
faster in COS7 cells than COS1 cells.
David L. Haviland, Ph.D. (haviland at KIDS.WUSTL.EDU) wrote:
: Does anyone have any direct experience with protein expression (cDNA
: constructs) in the COS cells? The Harvard manual states that COS-7's are
: best for this purpose however, as most know, the description of COS-1 and
: COS-7 cells are virtually identical in the ATCC catalog. My only test
: thus far has been a beta-gal vector that serves as a control for
: transfection (DEAE). Using this vector, transfection and expression are
: effectively the same as far as I can tell.
: Many thanks in advance.
: Any thoughts?
: + David L. Haviland, Ph.D. .***. .***. .***. +
: + Washington Univ. School of Med * | | | * * | | | * | | | * +
: + Dept. of Peds./Pulm. Box 8116 *| | | * * | | | * * | | | * +
: + One Children's Place * | | | * | | | * * | | | * +
: + St. Louis, MO 63110 '***' '***' '***' +
: + Internet:"haviland at kids.wustl.edu" +
: + Voice: 314-454-6076 FAX: 314-454-6076 +
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