COS-cells

Riekeltje Koedood rkoedood at bio.bu.edu
Wed Mar 6 16:16:36 EST 1996


As far as I can remember, COS cells are C127(?) cells transformed with SV40
large T Antigen, COS7 has more copies of T Antigen than COS1. Therefore, 
expression vectors carrying the SV40 origin of replication should replicate 
faster in COS7 cells than COS1 cells.

Marieke


David L. Haviland, Ph.D. (haviland at KIDS.WUSTL.EDU) wrote:

: Does anyone have any direct experience with protein expression (cDNA 
: constructs) in the COS cells?  The Harvard manual states that COS-7's are 
: best for this purpose however, as most know, the description of COS-1 and 
: COS-7 cells are virtually identical in the ATCC catalog.  My only test 
: thus far has been a beta-gal vector that serves as a control for 
: transfection (DEAE).  Using this vector, transfection and expression are 
: effectively the same as far as I can tell. 

: Many thanks in advance.
: Any thoughts?
: David

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