Help-no plasmid!!!

burklab at aecom.yu.edu burklab at aecom.yu.edu
Wed Mar 6 16:18:26 EST 1996


We had a similar problem in ourlab.  We assumed that the problem was due 
to leaky expression of the fusion protein which was toxic to DH5 alpha.  
To get around this problem we transformed into XL-1 blue cells 
(Stratagene) which has a tight lac repressor.  We experienced no problems 
after that.

Alan




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