anti-HA cross reaction?
ar229 at FreeNet.Carleton.CA
Wed Mar 6 13:28:55 EST 1996
I have recently been transfecting a HA-tagged construct into Cos7 cells
and looking for expression via immunofluorescence and an anti-HA MAb
(12CA5). When I do the transfections using DEAE dextran I get an
unexpected reaction in both mock transfected and tagged-construct
transfected cells. I see "rings" of staining in the cytoplasm - makes me
think of vacuoles of some kind. This reaction is specific for the DEAE
dextran method since I don't see it when electroporating. It is specific
for the anti-HA MAb since I don't see it with another Ab against the
untagged region of my protein. Any ideas?
Allison Haggarty ar229 at freenet.carleton.ca
mdah at musica.mcgill.ca
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