Expression of GST fusion

Gary T. Chou garychou at tucson.princeton.edu
Thu Mar 7 02:18:21 EST 1996


Hi, I'm using the GST fusion protein expression system and getting fairly
good expression.  However, when purifying the protein, a significant amount 
of the fusion protein is getting broken down, and I get an enormous band 
of GST+extra a.a. running at ~30kD (GST is 26kD) immediately after cell 
lysis.  I've used a wide array of protease inhibitors which seem to have
little effect.  Has anyone had any luck in getting rid of this band?

Thanks,

Gary




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