baculovirus co-infection

BIOTEC54 biotec54 at
Thu Mar 7 21:33:05 EST 1996

Does anyone have any "tricks" for obtaining good co-infections (and
subsequent good expression of both proteins within the infected cells)
other then adding high MOI's (>5 ea) for each virus.
Infecting at that high of an MOI for each virus...esp at large scale
(>10L) translates into adding 10-20% volume of virus into your cell stock.
I'm particularly interested in co-assembly of ind. sub-units (and mixing
and matching) so don't suggest placing both genes on the same virus.
Henry George

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