optimize mammalial cloning

Uwe Uwe.Mahr at RODGAU.NETSURF.DE
Thu Mar 7 19:22:42 EST 1996


is it possible to optimize a transfected cell-line (by G418-selection) by subcloning, i. e. to 
get a better receptor expession. I think if i got a monoclonal mammalial cell-line which is 
overexpressing a receptor gene, it could improve the expression of this gene, to make a 
subcloning by dilution. I think the cells are dividing over the time and are so not longer be 
monoclonal.



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