NH4Ac/EtOH ppt of DNA from PCR ?
dseegert at aol.com
Fri Mar 8 06:59:59 EST 1996
it's quite easy to do precipitation that way:
Add 1/5 volume of 10 M Nh4OAc,
if DNA amount is smaller than 1 ug I prefer to add 0.5 ul glykogene (10
brief vortex, add 2 to 3 volumes of 100% ethanol, vortex, incubate for 30'
night at -20 degrees. Then spin 10', 14000, 4 degrees, discard
one or two times with 70% ethanol, air dry pellet or use speed vac,
in desired solvent (I prefer water). Thats it!!!
Hope that helps
Dirk (LEO) Seegert, Ph.D.
Fraunhofer Institute Hannover
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